Description
Malt Extract Yeast Agar Peptone (MYPA)
What is MYPA?
MYPA Mix is the most nutritious gelatinous medium for cultivating, isolating, and manipulating fungi. This is due to the inclusion of peptone derived from soybeans, which supports vigorous mycelium growth and provides the most efficient medium for long-term maintenance of mushroom cultures (over a year). Uses of MYPA include:
- Growing mushroom mycelium for expansion into grain spawn
- Long-term storage
Why use Malt Extract Agar Yeast for growing mushroom cultures?
Malt Extract
Malt extract contains a high concentration of maltose, providing a suitable environment for the growth of yeast and mold due to its rich source of carbon, protein, and nitrogenous compounds used as energy for fungi growth.
Agar-Agar
Agar-Agar is extracted from various seaweeds, such as gracilaria and gelidium. It contains no animal ingredients and, due to its unique gel properties and stability, is used as a firming and thickening agent for Petri dish experimentation.
Yeast (S. Cerevisiae)
The strain species S. Cerevisiae is grown on sugar beet molasses, making it a natural source of readily assimilated amino acids and proteins. For mushrooms, this means a rich, supplemented diet of dietary fiber, Vitamin D, and B Complex (Thiamine, Riboflavin, Niacin, Pantothenic Acid, Folic Acid, Biotin, Vitamin B6, and Vitamin B12).
Peptone
Mycological peptone is derived from soybean plants, commonly used in liquid culture recipes and agar recipes, and also added to substrates as a supplement. Supplementation with peptone stimulates mycelium growth and dramatically improves the quality of the projected mycelium mat.
Not for human consumption
Quantity Specifications:
These quantities are measured based on 60*15mm size Petri dishes:
- 21 g makes 35 Agar Plates (use 500 ml of hot water)
- 42 g makes 70 Agar Plates (use 1000 ml of hot water)
- 63 g makes 105 Agar Plates (use 1500 ml of hot water)
- 84 g makes 140 Agar Plates (use 2000 ml of hot water)
- 168 g makes 280 Agar Plates (use 4000 ml of hot water)
Petri dishes are not included.
Instructions
- Dissolve your MYPA packet in the specified ratio of hot water (e.g., 1000 ml water for 42 g of MYPA) and stir thoroughly with a spoon. If you have a magnetic stirrer, it will mix the solution properly. The content needs to be poured slowly, not all at once.
- Autoclave or pressure cook for 20 minutes at 15 psi.
- Pour into Petri dishes when it reaches 50 degrees Celsius (approximately hot enough to hold the flask/jar with one hand), and let it set for 10 minutes. Do not stir the solution after removing it from the autoclave, as this would trap air bubbles.
- Once the Petri dishes are set, inoculate them and wrap them with parafilm. It is recommended to set the plates aside for a week to check for contamination.
- Petri dishes are best stored upside down to avoid condensation.
Don’t have a pressure cooker? No problem; message us, and we will send you detailed instructions on how to use a microwave for sterilizing along with your purchase. Please check our listing Make Agar Plates to see what you need. You would need a reagent bottle: a 500 ml reagent bottle if made in a microwave or a 1000 ml reagent bottle if using a sterilizer.
You will need a reagent bottle to make agar: a 500 ml reagent bottle if made in a microwave or a 1000 ml reagent bottle if using a sterilizer.
We rely on this mix to experiment with different varieties in our lab.
Check out our other listings to add liquid cultures and see more pre-mixes. We also sell other types of mushroom-growing equipment.
