Description
Make Your Own MYAG Agar Petri Dishes, Contamination-Free
General MYAG with Antibacterial Properties
This premium product is revolutionizing mushroom cultivation. MYAG is a gelatinous medium used to purify, germinate, isolate, and manipulate mushroom mycelium, drastically reducing contamination chances due to its antibacterial compound.
Why use MYAG instead of just MYA?
Most spores, whether obtained from the wild, homegrown, or trusted spore sellers, might include bacterial contamination due to methods of spore collection, preservation, and transportation (such as spore syringes and spore prints). In many cases, infected spores carry bacteria that introduce them to the agar medium, resulting in fast-growing bacterial spores that make it difficult for mushroom spores to germinate.
However, when infected spores are introduced to MYAG (with antibacterial properties), the mushroom spores will germinate and grow, leaving behind bacterial contamination. This is because the cells composing the mycelium (hyphae) have long, sharp filaments that carry reduced populations (if any) of bacteria when passing through the antibacterial properties. The antibacterial powder used is an antibiotic commonly used to treat bacterial infections and has been demonstrated to inhibit bacteria to a confinable level in agar mycelium cultures.
Uses of MYAG with Antibacterial Properties:
- Germinating spores from a spore syringe or spore print with a likelihood of contamination
- Purifying mycelium from other contaminated agar plates
- Cloning wild mushroom tissue, which has a likelihood of contamination
Quantity specifications:
These quantities are measured based on 60*15mm size Petri dishes:
The number of plates made will vary according to pouring techniques; we recommend using just enough to cover the surface of the petri dish.
- 21 gm makes 35 Agar Plates (Use 500ml of hot water)
- 42 gm makes 70 Agar Plates (Use 1000ml of hot water)
- 63 gm makes 105 Agar Plates (Use 1500ml of hot water)
- 84 gm makes 140 Agar Plates (Use 2000ml of hot water)
- 168gm makes 280 Agar Plates (Use 4000ml of hot water)
Instructions
- Dissolve your MYAG packet in the specified ratio of hot water (e.g., 1000 ml water for 42 g of MYAG) and stir thoroughly with a spoon. If you have a magnetic stirrer, it will mix the solution properly. The content needs to be poured slowly, not all at once.
- Autoclave or pressure cook for 15 minutes at 15 psi.
- Pour into Petri dishes when it reaches 50 degrees Celsius (approximately hot enough to hold the flask/jar with one hand), and let it set for 10 minutes. Do not stir the solution after removing it from the autoclave, as this would trap air bubbles.
- Once the Petri dishes are set, inoculate them and wrap them with parafilm. It is recommended to set the plates aside for a week to check for contamination.
- Petri dishes are best stored upside down to avoid condensation.
Don’t have a pressure cooker? No problem; message us, and we will send you detailed instructions on how to use a microwave for sterilizing along with your purchase. Please check our listing Make Agar Plates to see what you need. You would need a reagent bottle: a 500 ml reagent bottle if made in a microwave or a 1000 ml reagent bottle if using a sterilizer.
We rely on this mix to experiment with different varieties in our lab.
Check out our other listings to add liquid cultures and see more pre-mixes. We also sell other types of mushroom-growing equipment.
You can also buy antibacterial powder without yeast from our store.
